MicroRNA Risk Scores were calculated on the training set and area-under-the-curve receiver-operating-characteristic (AUC-ROC) curves were derived from the validation set. Real time reverse transcription quantitative polymerase chain reaction (rt-qPCR) was performed on each sample for 45 microRNAs. Samples were divided into training and validation sets. After sample quality was confirmed, 139 term deliveries (38-42 weeks) and 18 spontaneous preterm deliveries (<35 weeks) were selected for analysis. Using these entry criteria, 486 samples were selected for study inclusion. For initial study inclusion, samples were selected that met the following criteria: 1) singleton pregnancy 2) maternal body mass index (BMI) <30 kg/m2 3) blood sample drawn between 6 weeks to 12 weeks 6 days gestation 4) live born neonate with no detectable birth defects. ![]() STUDY DESIGN:This was a retrospective nested case-control study in pregnant patients enrolled between March 2006 and October 2016. OBJECTIVE:To predict spontaneous preterm birth among pregnant women in an African American population using first trimester peripheral blood maternal immune cell microRNA.
0 Comments
Leave a Reply. |